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Promega improm-ii reverse transcription kit
Improm Ii Reverse Transcription Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/improm-ii+reverse+transcription+kit/improm+ii+reverse+transcription+system/us12359287-483-9-13
Average 90 stars, based on 1 article reviews
improm-ii reverse transcription kit - by Bioz Stars, 2026-09
90/100 stars

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Article Title: Direct actions of growth hormone in rainbow trout, Oncorhynchus mykiss, skeletal muscle cells in vitro.
Article Snippet: RT reactions were performed using the Promega ImProm-II Reverse Transcription Kit (Promega, Madison, WI, USA) and prepared as follows: Oligo(dT)/RNA mix, MgCl2 (6.0 mM), ImProm-II 5× Reaction Buffer, dNTP (0.5 mM), Recombinant RNasin Ribonuclease Inhibitor and ImProm-II Reverse Transcriptase. cDNA reaction mixes were placed in the thermocycler and equilibrated at 25 ◦C for 5 min, then incubated at 42 ◦C for 1 h, then inactivated for 15 min at 70 ◦C, and then held at 4 ◦C.

Article Title: Properties and parameters of novel biodegradable metallic alloys
Article Snippet: Reverse transcription was performed using a commercially available kit (Improm-II Reverse Transcription Kit, Promega).

Article Title: Ccr4-not ubiquitin ligase signaling regulates ribosomal protein homeostasis and inhibits 40S ribosomal autophagy
Article Snippet: The ImProm-II reverse transcription kit (Promega) was used to synthesize cDNA from 1 μg of total RNA using the included oligo dT primers in a volume of 20 μl per the manufacturer’s direction.

Article Title: The mechanical regulatory role of ATP13a3 in osteogenic differentiation of pre-osteoblasts.
Article Snippet: Purpose: The process of osteogenic differentiation hinges upon the pivotal role of mechanical signals.. Previous studies found that mechanical tensile strain of 2500 microstrain (με) at a frequency of 0.5 Hz promoted osteogenesis in vitro.. However, the mechanism of the mechanical strain influencing osteogenesis at the cellular and molecular levels are not yet fully understood.

Article Title: Fibroblast Growth Factor 20 Attenuates Colitis by Restoring Impaired Intestinal Epithelial Barrier Integrity and Modulating Macrophage Polarization via S100A9 in an NF-κB Dependent Manner.
Article Snippet: RNA was reverse transcribed using Improm-II reverse transcription 555 kit (A5001, Promega, USA) for qPCR.

Article Title: Combining human liver ECM with topographically featured electrospun scaffolds for engineering hepatic microenvironment
Article Snippet: Then RNA was reversed-transcribed to complementary DNA (cDNA) using the Improm-II Reverse Transcription kit (Promega) according to the manufacturer’s instructions. cDNA samples were stored at -20 °C until use.

Synthesized:

Article Title: FGF21 protects against HFpEF by improving cardiac mitochondrial bioenergetics in mice.
Article Snippet: Total RNA was isolated from frozen left ventricle tissue by the Trizol Reagent (15596018, Invitrogen, USA). .. Complementary DNA was then synthesized from 500ng total RNA by reverse transcription with an Improm-II reverse transcription kit (A5001, Promega, USA) with random hexamer primers. .. Real-time quantitative polymerase chain reaction (qPCR) was performed with a real-time PCR system (CFX96, BioRad, USA) with specific primers described in Table S3.

Article Title: FGF21 protects against HFpEF by improving cardiac mitochondrial bioenergetics in mice
Article Snippet: Total RNA was isolated from frozen left ventricle tissue by the Trizol Reagent (15596018, Invitrogen, USA). .. Complementary DNA was then synthesized from 500 ng total RNA by reverse transcription with an Improm-II reverse transcription kit (A5001, Promega, USA) with random hexamer primers. .. Real-time quantitative polymerase chain reaction (qPCR) was performed with a real-time PCR system (CFX96, Bio-Rad, USA) with specific primers described in Table .

Reverse Transcription:

Article Title: FGF21 protects against HFpEF by improving cardiac mitochondrial bioenergetics in mice.
Article Snippet: Total RNA was isolated from frozen left ventricle tissue by the Trizol Reagent (15596018, Invitrogen, USA). .. Complementary DNA was then synthesized from 500ng total RNA by reverse transcription with an Improm-II reverse transcription kit (A5001, Promega, USA) with random hexamer primers. .. Real-time quantitative polymerase chain reaction (qPCR) was performed with a real-time PCR system (CFX96, BioRad, USA) with specific primers described in Table S3.

Article Title: FGF21 protects against HFpEF by improving cardiac mitochondrial bioenergetics in mice
Article Snippet: Total RNA was isolated from frozen left ventricle tissue by the Trizol Reagent (15596018, Invitrogen, USA). .. Complementary DNA was then synthesized from 500 ng total RNA by reverse transcription with an Improm-II reverse transcription kit (A5001, Promega, USA) with random hexamer primers. .. Real-time quantitative polymerase chain reaction (qPCR) was performed with a real-time PCR system (CFX96, Bio-Rad, USA) with specific primers described in Table .

Random Hexamer:

Article Title: FGF21 protects against HFpEF by improving cardiac mitochondrial bioenergetics in mice.
Article Snippet: Total RNA was isolated from frozen left ventricle tissue by the Trizol Reagent (15596018, Invitrogen, USA). .. Complementary DNA was then synthesized from 500ng total RNA by reverse transcription with an Improm-II reverse transcription kit (A5001, Promega, USA) with random hexamer primers. .. Real-time quantitative polymerase chain reaction (qPCR) was performed with a real-time PCR system (CFX96, BioRad, USA) with specific primers described in Table S3.

Article Title: FGF21 protects against HFpEF by improving cardiac mitochondrial bioenergetics in mice
Article Snippet: Total RNA was isolated from frozen left ventricle tissue by the Trizol Reagent (15596018, Invitrogen, USA). .. Complementary DNA was then synthesized from 500 ng total RNA by reverse transcription with an Improm-II reverse transcription kit (A5001, Promega, USA) with random hexamer primers. .. Real-time quantitative polymerase chain reaction (qPCR) was performed with a real-time PCR system (CFX96, Bio-Rad, USA) with specific primers described in Table .



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